Olszewski Lecture: Spatially Resolved Single-Cell Genomics & Cell Atlas of the Brain
La conf茅rence Olszewski du Neuro, cr茅茅e en 1986, rend hommage au Dr Jerzy Olszewski (1913鈥�1964), un neuroanatomiste et neuropathologiste pionnier. Invit茅 au Neuro par le Dr Wilder Penfield en 1948, Olszewski a collabor茅 脿 l鈥檕uvrage fondamental Cytoarchitecture of the Human Brain Stem, contribuant 脿 l鈥檃vancement de la compr茅hension des structures du tronc c茅r茅bral et 茅tablissant son h茅ritage dans le domaine des neurosciences.
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Xiaowei Zhuang听
Howard Hughes Medical Institute, Harvard University
Abstract: Cell and tissue functions arise from the coordinated activities of thousands of different genes. Understanding these functions requires imaging at the genome scale, which advances knowledge in many areas of biology, from the regulation of gene expression in cells to the development of cell fate and the organization of cell types in complex tissues. A single-cell genome-scale imaging method, multiplexed error-robust fluorescence in situ hybridization (MERFISH), was developed to enable spatially resolved single-cell transcriptomics, epigenomics, 3D-genomics, and functional genomics. This method allows for single-cell gene-expression profiling in intact tissues, facilitating the identification, spatial mapping, and functional investigation of distinct cell types within those tissues. Using this approach, molecularly defined, spatially resolved, and functionally annotated cell atlases of the brain have been created. This lecture will describe the MERFISH technology and its applications, with a particular focus on mapping the molecular, spatial, and functional organizations of distinct cell types in the brain.
