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Imaging single mRNA molecules to investigate neuronal protein homeostasis

Maria Vera Ugalde, McGill University
Tuesday March 15, 12-1pm
Zoom Link:聽

础产蝉迟谤补肠迟:听Attaining three-dimensional data at high throughput is a grand challenge in microscopy. We propose and demonstrate a novel solution to enable the collection of 3D positions in thousands of cells each minute by merging two technologies: point-spread-function engineering and imaging flow cytometry. We apply this approach to track nanoscale dynamics in cell populations responding to drug treatments and undergoing environmental changes with robust statistics. Finally, I will discuss future opportunities of deploying flow-based, single-cell techniques in tandem for elucidating cell mechanisms and in building sensitive diagnostic tools.